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. 2018 Sep 25;9:3918. doi: 10.1038/s41467-018-06289-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Intercellular translocation of sbZIP60 induces BiP3 expression in systemic tissues. a Confocal laser scanning microscopy of Col-0; pSHR-GFP, shr; pSHR-SHR-GFP, and bzip28/60; pSHR-sbZIP60-GFP at the primary root tips of 5-day-old transgenics reveals stele (St) accumulation of GFP, and stele and endodermis (En) distribution of SHR-GFP; noticeably, sbZIP60-GFP is localized in the stele, endodermis, cortex (Co) and epidermis (Ep). Similarly to SHR-GFP, sbZIP60-GFP is localized in nuclei (arrows). As also reported earlier²², we did not find SHR-GFP localization in the nuclei of the cortex and epidermis. Propidium iodide (PI) was used for counterstaining. Scale bar: 50 μm. b Expression of pBIP3:GUS in bzip28/60, Col-0 and bzip28/60;pSHR-sbZIP60-GFP seedlings grown vertically on half LS agar medium for 11 days. X-Gluc was used for histochemical staining to monitor GUS activity. Scale bar: 100 μm. c Longitudinal confocal optical sections of the regions along the primary root shown in b. Epidermis: Ep; Co: cortex; En: endodermis; St: stele. The indications upper, middle and lower refer to the a, b, and c zones indicated in panel b. Scale bar: 20 μm