Fig. 4.

Micro-grafting analyses support the existence of endogenous systemic UPR signals. a–c Plots of the mean values (dots) of the quantitative RT-PCR analyses of the transcripts of sbZIP60 (a) BiP3 (b) and bZIP28 (c) in scion and rootstock of 4-week-old grafted seedlings of wild-type and bzip28/60 backgrounds treated with DMSO or 0.5 μM Tm (Tunicamycin) for 48 h as described in Fig. 3a. The average of all the values is indicated by a black line. Error bars represent mean with 95% confidence interval among all grafts. Error bars above and below indicate the 95th and 5th percentiles. Average values significantly different from the values of wild-type (Col-0) and bzip28/60 self-grafts are indicated by red asterisks (*P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001, NS, nonsignificant; Mann-Whitney test). n, total number of grafted unions