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. 2018 Sep 25;8:14337. doi: 10.1038/s41598-018-32198-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heatmap indicating variant frequency differences between the reference genome YFV-74018 and isolates selected after passages on Ae. albopictus Manaus (A) and PNMNI (B). All 10 passages were deep sequenced: P1 to P5 were performed using homogenates of mosquito heads, and P6-S to P10-S with mosquito saliva. The intensity of black corresponds to the variant frequency. Only variants for which the frequency is above 20% are shown.