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. 2018 Sep 25;9:3915. doi: 10.1038/s41467-018-06380-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Lnk deficiency fully restores phenotypic HSCs in Fancd2^−/− mice. a CBC analysis of peripheral blood from WT, Fancd2^−/− (D2^−/−), Lnk−/− (L^−/−) and FancD2^−/−;Lnk−/− (D2^−/−L^−/−) mice. WBC: white blood cell. Each symbol represents an individual mouse; horizontal lines indicate mean frequencies; error bars indicate SE. b–d Quantification of various HSPC compartments by flow cytometry using SLAM marker scheme, Lin-Kit + Sca1 + (LSK) HSPC population (b), MPP (CD48 + CD150-LSK) (c), HSC (CD48-CD150 + LSK), and LT-HSC (CD48-CD150 + Flk2-CD34-LSK) (d). e Quantification of various HSPC subsets using the CD34 and Flk2 surface marker scheme, HSCs (CD34-Flk2-LSK) and MPP (CD34 + Flk2 + LSK). f Quantification of various HSC and MPP subsets using indicated markers. LT-HSC: CD150 + CD48-Flk2-LSK; ST-HSC: CD150-CD48-Flk2-LSK; MPP1: CD150 + CD48-Flk2-CD34 + LSK; MPP2: CD150 + CD48 + Flk2-LSK; MPP3: CD150-CD48 + Flk2-LSK; MPP4: Flk2 + LSK. P values determined by two-tailed Student’s t-test are shown, *p < 0.05, **p < 0.01, ***p < 0.001, ns not significant. Data are pooled from 3–5 independent experiments