Skip to main content
. 2018 Sep 19;9:2198. doi: 10.3389/fmicb.2018.02198

Table 2A.

Sequence of primers used in DGGE analyses.

Microbial group Primer set Sequence Reference
Total bacteria EUBf933-GC EUBr1387 5′-GCA CAA GCG GTG GAG CAT GTG G-3′ 5′-GCC CGG GAA CGT ATT CAC CG-3′ Iwamoto et al., 2010
Actinobacteria f243 r513-GC 5′-GGA TGA GCCCGCGGCCTA-3′ 5′-gc-CGG CCG CGG CTG CTG GCA CGTA-3′, GC-clamp: CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG G Heuer et al., 1997
Total fungi fNS1 rNS8 NS7-GC f1r 5′-GTA GTC ATA TGC TTG TCT C-3′ 5′-TCC GCA GGT TCA CCT ACG GA-3′ 5′-GAG GCA ATA ACA GGT CTG TGA TGC-3, GC-clamp: CGC CCG GGG CGC GCC CCG GGC GGG GCG GGG GCA CGG GGG 5′-CTT TTA CTT CCT CTA AAT GAC C-3′ Cea et al., 2010
Ascomycete ITS4asco ITS3-GC 5′-CGT TAC TRR GGC AAT CCC TGT TG-3′ 5′-gc-GCA TCG ATG AAG AAC GCA GC-3′, GC-clamp: CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGGG Nikolcheva and Bärlocher, 2004; White et al., 1990