Table 1.

Unique cross-linking sites between GCAP-2 and ROS-GC peptides 1-3 in the presence (1 mM CaCl₂) and absence (10 mM EGTA) of calcium using DSBU, CDI, DAU, and photo-Met (PM) as cross-linkers.

Site (1)	Site (2)	Calcium
Peptide	mGCAP-2	With	Without
PEPTIDE 1
C-term	PM42		✔
N-term	K126	✔	✔
N-term	K128/129	✔	✔
N-term	K178	✔	✔
N-term	K200	✔	✔
N-term	K29/30	✔	✔
N-term	K46	✔	✔
N-term	K50	✔	✔
N-term	K96	✔	✔
N-term	K142	✔	✔
PEPTIDE 2
C5	C131	✔
D9	PM186	✔	✔
E18	PM181	✔	✔
M1	PM181	✔	✔
N-term	K102	✔	✔
N-term	K106	✔	✔
N-term	K128/129		✔
N-term	K142	✔	✔
N-term	K178	✔	✔
N-term	K200	✔	✔
N-term	K29/30	✔	✔
N-term	K46		✔
N-term	K50	✔	✔
N-term	K96		✔
N-term	K98	✔	✔
N-term	S37		✔
N-term	Y125/K126	✔	✔
S19	K200	✔	✔
S19	K46	✔	✔
S19/T20	K102	✔	✔
S19/T20	K129	✔
S19/T20	K29	✔
S19/T20/Y24	K200	✔	✔
Y24	K102		✔
Y24	K126	✔	✔
Y24	K178	✔
Y24	K50	✔	✔
Y4	K106		✔
Y4	K126	✔	✔
Y4	K29/30	✔	✔
Y4	K46	✔
Y4	K96/98	✔	✔
PEPTIDE 3
N-term	K126	✔
N-term	K128/129		✔
N-term	K178	✔
N-term	K200	✔	✔
N-term	K29/30	✔	✔
N-term	K46	✔	✔
N-term	K50		✔
N-term	K96	✔	✔
N-term/Y2	K98	✔
Y2	K106	✔	✔
Y2	K142		✔