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. 2018 Jul 2;17(5):e12800. doi: 10.1111/acel.12800

Figure 3.

Figure 3

Spontaneous intervertebral disk degeneration in skeletally mature mice with conditional deletion of FOXO. (a) Safranin O staining in lumbar intervertebral disk (IVD) from AcanCreER−/− and AcanCreER‐FOXO KO mice at 6 and 12 months of age (n = 6–8 mice per group). NP: nucleus pulposus; AF: annulus fibrosus; EP: endplate. Magnification bar = 100 µm. (b) Quantification of cellularity in NP, AF, and EP of lumbar IVD from AcanCreER−/− and AcanCreER‐FOXO KO mice at 6 and 12 months of age (n = 6–8 mice per group). (c) Measurement of disk height of lumbar IVD samples AcanCreER−/− and AcanCreER‐FOXO KO mice at 6 and 12 months of age (n = 6–8 mice per group). (d) Histopathological scores in the NP/AF and EP of lumbar IVD from AcanCreER−/− and AcanCreER‐FOXO KO mice at 6 and 12 months of age (n = 6–8 mice per group). Values shown are mean ± SD. Statistical comparisons were assessed by an unpaired, two‐tailed t‐test after testing for equal variance using an F‐test. Values are mean ± SD. *p < 0.05