Figure 4.

Electrophysiological recordings of human circulating monocytes transdifferentiated into neural-like cells. (A) Light microscopy photographs of two representative neuronal-like cells in which electrical activity was found. Scale bars = 20 μm. (B) Recordings under current clamp conditions showing spontaneous action potentials (APs) on a transdifferentiated neuronal-like cell. (C) Recordings under current clamp conditions showing spontaneous excitatory postsynaptic potentials (EPSPs) and inhibitory postsynaptic potentials (IPSPs) on a transdifferentiated neuronal-like cell. (D) Average of 134 APs present in one transdifferentiated neuronal-like cell. (E) Average of 45 EPSPs present in one transdifferentiated neuronal-like cell. (F) Average of 34 IPSPs present in the same transdifferentiated cell shown in (E). Of note, over 200 recordings were obtained from 11 cells from five different healthy individuals.