Figure 1.

Typical survival plot for C. elegans glp‐1 strain at 25°C in our laboratory. Data show survival data for n = 97 nematodes from a cohort maintained at 25°C. Significant increases in morbidity and cumulative mortality were observed after Day 10. To avoid confounders such as dead worms and selection effects during the mutation capture experiments, cohorts were sampled on Days 4 (three cohorts), 7 (five cohorts), and 10 (three cohorts) for mtDNA extraction