Table 2.
Summary of validated DGF‐specific targets in the RNAseq cohort, the validation cohort (directionality of changes indicated by arrows)
| RNAseq cohort (IGF = 11, extreme DGF = 8) | Validation cohort (IGF = 7, DGF = 9) | Combined cohort (Overall DGF = 17; no DGF = 34) | DCD vs. DBD | ECD vs. SCD | Correlations (correlation coefficient; p‐value) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Donor age | MDRD4 at 3 months | MDRD4 at 6 months | MDRD4 at 12 months | CIT | WIT | Cr T1/2 | |||||
| ACKR3 Pre (p = 0.02)↓ | C1QB Pre (p = 0.029) | ABCA8 Pre (p = 0.017)↑ | REG1A (p = 0.026)↓ | CXCL9 Pre (p = 0.009)↑ | TAGAP Pre (0.46; 0.001) | PTPRC Pre (−0.35; 0.000) | PTPRC Pre (−0.45; 0.001) | GABBR1 Pre (−0.35; 0.028) | ACKR3 Pre (0.33; 0.030) | GABBR1 Post (−0.32; 0.031) | SEMA3A Post (0.33; 0.025) |
| BTN3A2 Post (p = 0.041)↑ | FCGR1C Post (p = 0.031) ↑ | ACKR3 Pre (p = 0.007)↓ | CHGB Post (p = 0.015)↓ | UBD pre (p = 0.036)↑ | PTPRC Pre (0.5; 0.000) | PTPRC Post (−0.34; 0.014) | PTPRC Post (−0.34; 0.020) | ACKR3 Post (−0.37; 0.019) | CHGB Pre (0.35; 0.010) | CORIN Post (−0.29; 0.042) | CHGB Post (−0.27; 0.047) |
| CCL19 Post (p = 0.041)↑ | FCGR1C Pre (p = 0.001)↑ | BTN3A2 Post (p = 0.038)↑ | CHGB Pre (p = 0.021)↓ | TAGAP Pre (p = 0.001)↑ | NLRC4 Pre (0.32; 0.024) | NLRC4 Pre (−0.34; 0.014) | NLRC4 Pre (−0.48; 0.000) | ZNF676 Post (−0.31; 0.043) | REG1B Pre (0.38; 0.006) | ||
| CD52 Pre (p = 0.021)↑ | KLRB1 Pre (p = 0.020) ↑ | CD52 Pre (p = 0.029)↑ | CORIN Post (p = 0.001)↓ | PTPRC Pre (p = 0.001)↑ | KLRB1 Pre (0.36; 0.010) | KLRB1 Pre (−0.30; 0.033) | KLRB1 Pre (−0.41; 0.003) | ||||
| CHGB Post (p = 0.008)↓ | NLRC4 Pre (p = 0.043)↑ | CHGB Post (p = 0.012)↓ | REG1B Post (p = 0.026)↓ | KLRB1 Pre (p = 0.028) ↑ | CD52 Pre (0.38; 0.007) | KLRB1 Post (−0.28; 0.045) | KLRB1 Post (−0.31; 0.023) | ||||
| CHGB Pre (p = 0.01)↓ | OAS2 Post (p = 0.045)↑ | CXCL10 Post (p = 0.039)↑ | FCGR1C Pre (p = 0.001)↑ | CD69 Pre (p = 0.008) ↑ | CCL19 Pre (0.34; 0.014) | CD52 Pre (−0.32; 0.024) | CD52 Pre (−0.32; 0.024) | ||||
| CXCL10 Post (p = 0.015)↑ | PTPRC Post (p = 0.029)↑ | FAM34A Pre (p = 0.012)↓ | TRIM Pre (p = 0.031)↑ | CD52 Pre (p = 0.014) ↑ | SEMA3A Pre (0.41; 0.007) | CCL19 Post (−0.34; 0.014) | CCL19 Post (−0.32; 0.021) | ||||
| CORIN Pre (p = 0.017)↓ | PTPRC Pre (p = 0.020)↑ | FCGR1C Post (p = 0.020)↑ | TRIM Post (p = 0.003)↑ | CCL19 Pre (p = 0.026) ↑ | SEMA3A Post (0.33; 0.028) | CHGB Pre (−0.274; 0.043) | SEMA3A Pre (0.43; 0.003) | ||||
| DISP2 Post (p = 0.013)↑ | SEMA3A Post (p = 0.018)↑ | FCGR1C Pre (p = 0.009) ↑ | RSPO1 Pre (p = 0.013)↑ | C1QB Pre (p = 0.033) ↑ | UBD Pre (0.32; 0.020) | SEMA3A Post (−0.36; 0.016) | SEMA3A Post (−0.36; 0.014) | ||||
| DISP2 Pre (p = 0.02)↑ | GABBR1 Post (p = 0.037)↑ | KLRB1 Post (p = 0.007)↓ | ISG20 Pre (p = 0.045)↑ | FCGR1C Pre (0.2; 0.022) | GABBR1 Post (−0.32; 0.031) | NLRP2 Post (−0.30; 0.041) | |||||
| FAM34A Pre (p = 0.043)↓ | KLRB1 Pre (p = 0.026)↑ | FAM34A Pre (p = 0.028)↓ | LLCIOB Post (p = 0.040)↑ | GABBR1 Pre (−0.33; 0.028) | |||||||
| FCGR1C Post (p = 0.041)↑ | NLRC4 Pre (p = 0.018)↑ | CXCL10 Pre (p = 0.03)↑ | GABBR1 Post (−0.34; 0.019) | ||||||||
| FCGR2C Post (p = 0.033)↑ | NLRP2 Pre (p = 0.025)↓ | ABCA8 Pre (p = 0.001)↑ | CORIN Post (−0.32; 0.018) | ||||||||
| FCRL3 Pre (p = 0.013)↓ | OAS2 Post (p = 0.017)↑ | ABCA8 Post (p = 0.026)↑ | FCGR2C Pre (−0.33; 0.017) | ||||||||
| FLG Pre (p = 0.042)↓ | PTPRC Pre (p = 0.014)↑ | BTN3A2 Pre (p = 0.019)↑ | FCGR1C Pre (−0.32; 0.018) | ||||||||
| KLRB1 Pre (p = 0.026)↑ | SEMA3A Post (p = 0.002)↑ | BTN3A2 Post (p = 0.04)↑ | |||||||||
| OAS Post (p = 0.027) ↑ | TAGAP Post (p = 0.01)↑ | OAS2 Pre (p = 0.000)↑ | |||||||||
| REG1B Post (p = 0.043)↑ | ZNF676 Pre (p = 0.032)↓ | OAS2 Post (p = 0.006)↑ | |||||||||
| REG1B Pre (p = 0.034)↑ | ISG20 Pre (p = 0.045)↓ | ||||||||||
| NLRP2 Pre (p = 0.013)↓ | ISG20 Post (p = 0.031)↓ | ||||||||||
| UBD Post (p = 0.006)↑ | INFG Post (p = 0.032)↑ | ||||||||||
| UBD Pre (p = 0.013)↑ | LLCIOB Pre (p = 0.002)↓ | ||||||||||
| SEMA3A Post (p = 0.004)↑ | LLCIOB Post (p = 0.014)↓ | ||||||||||
| TAGAP Post (p = 0.021)↑ | |||||||||||
| ZNF676 Pre (p < 0.0001)↓ | |||||||||||
The Mann–Whitney U test was used to compare variables in relation to different categories analysed. The FDR adjustment for multiple comparisons was performed and all targets retained significance (p < 0.05). Spearman correlation was used to analyse DGF‐specific targets with donor age, CIT, WIT, Cr T1/2 and MDRD4 at 3, 6 and 12 months post‐transplant. The table presents unadjusted p‐values.
CIT: cold ischaemic time; Cr T1/2: time of reduction in serum creatinine by 50%; DBD: donation after brain death; DCD: donation after cardiac death; ECD: extended criteria donor; IGF: immediate graft function no DGF includes allografts with primary function; MDRD4: estimated glomerular filtration rate modification of diet in renal disease (MDRD) equation; SCD: standard criteria donor; WIT: warm ischaemic time.