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. 2018 Sep 25;9:3911. doi: 10.1038/s41467-018-06388-7

Table 3.

Principal parameters from free fluctuation analysis after compound injection

AEG AEG QUI QUI GAM GAM
TTX+ state
 Sample size 10 10 14 15 12 7
 Best frequency (Hz) 196.90 (3.09) 445.33* (29.55) 210.58 (4.54) 317.08*** (7.67) 215.00 (6.64) 339.63** (6.51)
 Tuning sharpness Q 2.76 (0.45) 68.13** (58.90) 16.01* (4.89) 156.88** (290.01) 1.21 (0.06) 245.76** (945.39)
TeNT+ state
 Sample size 11 11 15 14 12 8
 Best frequency (Hz) 199.27 (2.05) 353.04** (25.09) 222.44 (6.39) 301.21*** (7.32) 218.70 (3.51) 327.01*** (8.90)
 Tuning sharpness Q 2.55 (0.24) 96.14*** (557.70) 14.07* (6.98) 309.65*** (162.60) 0.99 (0.16) 3034.38*** (1374.67)
Combined male induced SO state
 Sample size 21 29 15
 Best frequency (Hz) 384.43 (20.45) 311.23 (5.52) 332.04 (5.81)
 Tuning sharpness Q 93.05 (296.39) 239.28 (166.47) 355.79 (895.09)
Power gain Ringer (kBT) 1.84 (0.52) 3.61 (1.68) 15.35 (4.88) 7.47 (5.48) 1.72 (0.24) 3.78 (0.83)
Power gain TTX (kBT) 1.56 (0.40) 45.43** (14.62) 24.63* (4.38) 722.72*** (6004.67) 1.42 (0.31) 19280.68** (14592.23)
Power gain TeNT (kBT) 1.56 (0.25) 66.57** (99.16) 21.89* (9.20) 1078.9*** (2055.62) 1.55 (0.45) 44934.32*** (469402.29)
Power gain-induced SO state (kBT) 49.70 (19.87) 1041.56 (3218.82) 40134.86 (254561.08)
Power gain pymetrozine (kBT) 0.15*** (0.08) 0.15*** (0.16) 0.17*** (0.15) 0.19*** (0.07) 0.12*** (0.12) 0.15*** (0.07)

Median values obtained from harmonic oscillator fits for female and male Ae. aegypti (AEG), Cx. quinquefasciatus (QUI) and An. gambiae (GAM) following TTX or TeNT injection (standard errors are given in brackets); these include the best frequency and tuning sharpness (Q) of the flagellum. Combined median values including both TTX and TeNT injected states are shown to present data for all males exhibiting induced self-sustained oscillations (SOs). Median values of the power gain in four injection states (after Ringer, TTX, TeNT or pymetrozine injection), as well as a value for the combined induced state (including both TTX and TeNT), are given with corresponding standard errors. Significant differences in best frequency/Q/power gain between the control (Ringer) state and any other state for a specific mosquito group are starred (repeated-measures ANOVA on ranks; *p < 0.05; **p < 0.01; ***p < 0.001). No significant differences in best frequency/Q/power gain between the TTX exposed and TeNT exposed states for a specific mosquito group were calculated (ANOVA on ranks with a significance level of p < 0.05)