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. 2018 Sep 25;8:14351. doi: 10.1038/s41598-018-32713-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(Upper left) Comparison of SNR Gains for PCA and MNF denoising with a different number of bands taken for reconstructions for an experimental whole tissue core shown in Fig. 7. (Bottom row) Single spectra taken from the same experimental pixel from Fig. 7. (Upper right) Actual SNR values of an experimental 2 to 256 scans of a single 64 × 64 tile of a tissue compared with PCA and MNF denoised values of the same image, showing the clear gain from denoising regardless of the actual number of scans.