Fig. 5. A1 treatment protects retinal neurons, and increases microglia/ macrophages after IR injury.

a Mice received intravitreal injection of PEG-A1 (1.7 ng/µl) 3 h before induction of IR injury and were sacrificed at day 7. PEG-A1 treatment preserved retinal neurons (NeuN-positive cells), n = 6 for PBS and 5 for PEG-A1, *p < 0.05. b PEG-A1-treated retinas showed increased microglia/macrophage infiltration as evident by Iba1 staining on retina flat-mounts. c Treatment with PEG-A1 (1.7 ng/µl) 3 h after IR injury achieved similar neuronal preservation to the pretreatment, thus showing post-injury protective effect, n = 5 for PBS and 7 for PEG-A1, *p < 0.05