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. 2018 Sep 25;8:14359. doi: 10.1038/s41598-018-32523-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell culture validation on the breathing chip. Experimental handling of the new lung-on-chip array was tested with a commercially available viability test and the 16HBE14o- cell line. Breathing mode (dynamic) was started on day 3. 16HBE14o- cells demonstrate under stretch a slightly increased viability (n = 3).