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. 2018 Sep 25;8:14322. doi: 10.1038/s41598-018-32774-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of pbsP is upregulated in vivo. (A) RT-PCR analysis of pbsP mRNA levels in bacteria grown in Todd-Hewitt broth (TH) or isolated from the kidneys and brains of BM110-infected mice. Values are presented as ratios relative to the values observed in bacteria grown in TH broth. Results are means ± SD from four independent experiments performed in triplicate. (B) RT-PCR analysis of pbsP mRNA levels in wild-type CC17 BM110 strain (BM110), wild-type CC23 NEM316 strain (NEM316) and their respective CovR deletion mutants (BM110 ΔCovR and NEM316 ΔCovR). Values were normalized for those observed in wild-type BM110. Results are means ± SD from three independent experiments performed in triplicate. *p < 0.05; ***p < 0.001 by Student’s t test; ns, non-significant.