Fig. 5.

Mutation of the Arg and Gln fingers of the TBC-like motif of Etf-2 impairs Etf-2 localization to RAB5-CA endosomes. (A) RF/6A cells were cotransfected with HA-RAB5-CA and with Etf-2^R188A (Etf-2^RA), Etf-2^Q245A (Etf-2^QA), Etf-2^R188A/Q245A (Etf-2^DM), or Etf-2-GFP. At 2 dpt, cells were subjected to immunofluorescence labeling with mouse anti-HA (red; AF555). Merged/DIC, the fluorescence image is merged with the DIC image. Boxed areas are enlarged 4× on the right. (Scale bars: 10 μm.) (B) Quantification of 100–120 RAB5-CA endosomes in 20–30 cotransfected RF/6A cells from three independent experiments. *P < 0.05, ANOVA.