Fig. 6.

Hizentra decreases survival of activated CLL cells from group 2 patients. Purified CLL cells were cultured in AIM-V serum-free media in the presence or absence of anti-IgM antibodies with or without Hizentra at a final concentration of 5 or 15 g/L. Percentages of viable cells that exclude the dye 7AAD were measured by flow cytometry after 5 days. A. Examples are shown in the dot-plots with the numbers representing percentages of viable 7AAD⁻ cells. B. The summary graph shows the average and standard error of the relative survival of 6 Group 1 patient samples and 4 group 2 samples. Relative survival is defined as the percentage of 7AAD⁻ cells with Hizentra divided by the percentage in control cultures without Hizentra. Groups 1 and 2 represent CLL cells that spontaneously make > or <38 pg/ml of TNFα, respectively. The results indicate group 2 samples are most sensitive to Hizentra, especially at 15 g/L. C. Schematic diagram suggesting that high-normal IgG levels may help control BCR-signaling in CLL cells. *;p < 0.05.