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SPR sensorgrams of FGF2-heparin interaction at different temperatures, A: 10°c; B: 25°c; C: 30°c. Concentrations of FGF2 (from top to bottom): 200, 100, 50, 25, and 12.5 nM, respectively. The black curves are the fitting curves using 1:1 Langmuir binding model from BIAevaluate 4.0.1.
