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. Author manuscript; available in PMC: 2018 Sep 26.
Published in final edited form as: Biochem Physiol. 2018 Jul 31;7(2):241. doi: 10.4172/2168-9652.1000241

Table 1:

Summary of kinetic data of heparin-protein interaction at different temperatures.

Protein Temperature ka (1/MS) kd (1/S) Kd (M)
ATIII 10°C ka1 = 2.63 × 105 (± 437) (1/MS)
ka2 = 0.021(± 3.7 × 10−5) (1/S)
kd1 = 0.047 (± 1.0 × 10−4) (1/S)
kd2 = 4.78 × 10−4 (± 1.1 × 10−6) (1/S)
25°C ka1 = 2.4 × 10s (± 433) (1/MS)
ka2 = 0.024 (± 5.1 × 10−5) (1/S)
kd1 = 0.043 (± 1.1 × 10−4) (1/S)
kd2 = 2.34 × 10−4 (± 6.7 × 10−7) (1/S)
30°C ka1 = 1.9 × 105 (± 886) (1/MS)
ka2 = 0.019 (± 8.1 × 10−5) (1/S)
kd1 = 0.035 (± 2.4 × 10−4) (1/S)
kd2 = 7.9 × 10−7(± 5.3 × 10−9) (1/S)
FGF1 10°C 9.1 × 104
(± 6.6 × 103)
7.6 × 10−3
(± 4.7 × 10−5)
8.3 × 10−8
25°C 2.0 × 105
(± 6.6 × 103)
0.017
(± 4.7 × 10−5)
8.3 × 10−8
30°C 2.0 × 105
(± 6.6 × 103)
0.018
(± 4.7 × 10−5)
1.1 × 10−7
FGF2 10°C 4.6 × 107
(± 6.6 × 103)
0.102
(± 4.7 × 10−5)
2.2 × 10−9
25°C 3.8 × 108
(± 6.6 × 103)
0.406
(± 4.7 × 10−5)
1.1 × 10−9
30°C 9.2 × 107
(± 6.6 × 103)
0.296
(± 4.7 × 10−5)
3.2 × 10−9