Table I.
Peptides used in this Study and their Calculated Theoretical Isoelectric Point and Hydropathicity
| Peptide ID | Peptide sequence NBD-G-XXXXXXXXXXX-SIINFEKLAAK |
Theoretical pI* | Hydropathicity (GRAVY)** |
|---|---|---|---|
| SLP 1 | DEDKDKDDEEA | 3.95 | −1.208 |
| SLP 2 | DEEEKEGKEKA | 4.51 | −1.096 |
| SLP 3 | RKDDKDDKDLA | 6.67 | −0.962 |
| SLP 4 | RKHDHEHEHHA | 7.93 | −1.171 |
| SLP 5 | EDKKKSEKESA | 9.28 | −1.017 |
| SLP 6 | DEKRKKERELA | 9.42 | −1.004 |
| SLP 7 | DELYDLYDELA | 4.13 | −0.079 |
| SLP 8 | DEGLLRHLDEA | 4.99 | −0.163 |
| SLP 9 | DAKHDHLLHAA | 7.53 | −0.104 |
| SLP 10 | LDKKLLEKELA | 8.61 | −0.008 |
| SLP 11 | RIDIRLIIEIA | 8.83 | 0.713 |
| SLP 12 | GSAAESASGSA | 6.53 | 0.126 |
| SLP 13 | RDKSLKELLSA | 9.54 | −0.113 |
| SLP 14 | ELIDIIDIEIA | 4.25 | 0.796 |
| SLP 15 | DLKLADLLALA | 6.44 | 0.771 |
*The pI was calculated for the unlabeled peptide of which the N-terminus was not blocked, so the real theoretical pI values are expected to be slightly lower for the labeled peptide
**The sum of hydropathy values of all amino acids, divided by the number of residues in the sequence (the NBD label was not taken into account.). SLPs with a GRAVY score < 0 are relatively hydrophilic and SLPs with a GRAVY score > 0 are relatively hydrophobic (12,15)