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. 2018 Jul 14;45(5):1055–1065. doi: 10.1007/s11033-018-4254-0

Table 1.

Oligonucleotides used in the study

Primer Sequence (5′ → 3′) Locus Ta [°C] PCR [bp] Ref.
TEM-A
TEM-B
ATAAAATTCTTGAAGAC
TTACCAATGCTTAATCA
Flank of blaTEM-like genes 42 1181 [12]
P1C
P2D
TTAATTCGTCTCTTCCAGA
CAGCGCTTTTGCCGTCTAAG
Flank of blaCTX-M1-like genes 55 1042 [11]
SHV-A
SHV-B
ACTGAATGAGGCGCTTCC
ATCCCGCAGATAAATCACC
Flank of blaSHV-like genes 55 329 [12]
OXA-1/F
OXA-1/R
ATGAAAAACACAATACATATCAAC
TTTCCTGTAAGTGCGGACAC
Internal fragment of blaOXA-1-related genes 48 755 [35]
CF-1
CF-2
ATGATGAAAAAATCGATATG
TTATTGCAGTTTTTCAAGAATG
Flank of blaCMY-like genes 45 1146 [15]
aac(3)-IIF
aac(3)-II
TGAAACGCTGACGGAGCCTC
GTCGAACAGGTAGCACTGAG
aac(3)-II 55 369 [36]
sul1-F
sul1-R
TGGTGACGGTGTTCGGCATTC
GCGAAGGTTTCCGAGAAGGTG
sulI 56 790 [37]
SUL2F
SUL2R
CGGCATCGTCAACATAACCT
TGTGCGGATGAAGTCAGCTC
sulII 55 721 [38]
SUL3F
SUL3R
CAACGGAAGTGGGCGTTGTGGA
GCTGCACCAATTCGCTGAACG
sulIII 57 244 [38]
gyrA-P1
gyrA-P3
TGT CCG AGA TGG CCT GAA GC
TGC CGT CAT AGT TAT CAA CGA
QRDR gyrA 58 374 [37]
parC-3
parC-4
CCG TGC GTT GCC GTT TAT TG
AAGTGCCGTCGAAGTTTGGCA
QRDR parC 58 368 [37]
qnrA-1
qnrA-2
ATTTCTCACGCCAGGATTTG
GATCGGCAAAGGTTAGGTCA
qnrA Gradient 516 [39, 40]
qnrB-1
qnrB-2
GATCGTGAAAGCCAGAAAGG
ACGATGCCTGGTAGTTGTCC
qnrB 469
qnrS-1
qnrS-2
ACGACATTCGTCAACTGCAA
TAAATTGGCACCCTGTAGGC
qnrS 417

Ta annealing temperature of PCR, Ref references, bp base pair