Table 1.
Primer | Sequence (5′ → 3′) | Locus | Ta [°C] | PCR [bp] | Ref. |
---|---|---|---|---|---|
TEM-A TEM-B |
ATAAAATTCTTGAAGAC TTACCAATGCTTAATCA |
Flank of blaTEM-like genes | 42 | 1181 | [12] |
P1C P2D |
TTAATTCGTCTCTTCCAGA CAGCGCTTTTGCCGTCTAAG |
Flank of blaCTX-M1-like genes | 55 | 1042 | [11] |
SHV-A SHV-B |
ACTGAATGAGGCGCTTCC ATCCCGCAGATAAATCACC |
Flank of blaSHV-like genes | 55 | 329 | [12] |
OXA-1/F OXA-1/R |
ATGAAAAACACAATACATATCAAC TTTCCTGTAAGTGCGGACAC |
Internal fragment of blaOXA-1-related genes | 48 | 755 | [35] |
CF-1 CF-2 |
ATGATGAAAAAATCGATATG TTATTGCAGTTTTTCAAGAATG |
Flank of blaCMY-like genes | 45 | 1146 | [15] |
aac(3)-IIF aac(3)-II |
TGAAACGCTGACGGAGCCTC GTCGAACAGGTAGCACTGAG |
aac(3)-II | 55 | 369 | [36] |
sul1-F sul1-R |
TGGTGACGGTGTTCGGCATTC GCGAAGGTTTCCGAGAAGGTG |
sulI | 56 | 790 | [37] |
SUL2F SUL2R |
CGGCATCGTCAACATAACCT TGTGCGGATGAAGTCAGCTC |
sulII | 55 | 721 | [38] |
SUL3F SUL3R |
CAACGGAAGTGGGCGTTGTGGA GCTGCACCAATTCGCTGAACG |
sulIII | 57 | 244 | [38] |
gyrA-P1 gyrA-P3 |
TGT CCG AGA TGG CCT GAA GC TGC CGT CAT AGT TAT CAA CGA |
QRDR gyrA | 58 | 374 | [37] |
parC-3 parC-4 |
CCG TGC GTT GCC GTT TAT TG AAGTGCCGTCGAAGTTTGGCA |
QRDR parC | 58 | 368 | [37] |
qnrA-1 qnrA-2 |
ATTTCTCACGCCAGGATTTG GATCGGCAAAGGTTAGGTCA |
qnrA | Gradient | 516 | [39, 40] |
qnrB-1 qnrB-2 |
GATCGTGAAAGCCAGAAAGG ACGATGCCTGGTAGTTGTCC |
qnrB | 469 | ||
qnrS-1 qnrS-2 |
ACGACATTCGTCAACTGCAA TAAATTGGCACCCTGTAGGC |
qnrS | 417 |
Ta annealing temperature of PCR, Ref references, bp base pair