(A) Publicly available (GEO) microarray datasets relating to ulcerative colitis (UC) and Crohn’s disease (CD) were analyzed for an over-representation of a Keap1-inhibition signature gene set assembled from differential expression analysis of RNA sequencing (RNA-seq) data (Figure 2). In 3 of the 4 IBD datasets analyzed, Keap1-inhibition genes were over-represented in comparisons of diseased versus healthy and inflamed versus uninflamed tissue, with the most significant results appearing in comparisons of UC patients with healthy controls. The genes contributing to these overlaps are presented as a word cloud with the size of the word denoting how many public datasets these genes were found enriched.
(B–D) Z-transformed expression values for Keap-1 inhibition signature genes also differentially expressed in GSE59071 (Vanhove et al., 2015) CD samples (B) or UC samples (C). Z-transformed expression values for Keap-1 inhibition signature genes also differentially expressed in GSE38713 (Planell et al., 2013) (D).