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. Author manuscript; available in PMC: 2018 Sep 26.
Published in final edited form as: Am J Med Genet A. 2003 Apr 1;118A(1):35–42. doi: 10.1002/ajmg.a.10011

Fig. 4.

Fig. 4.

DNA sequence chromatograms from a control (a) and an affected individual (b) from pedigree DEN9 showing the point mutation in exon 2 of the PAX9 gene on the non-coding strand. c: Segregation of the mutation in DEN9. The mutation in DEN9 destroyed a cleavage site for MspA1. Amplification of a 214-bp PCR product with primers hPAX9ex2a2F and hPAX9ex2a1R was followed by digestion with MspA1. Digestion of the product with MspA1 resulted in a 189-bp product and a 25-bp product (not visible) in unaffected individuals.