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. Author manuscript; available in PMC: 2018 Sep 26.
Published in final edited form as: Am J Med Genet A. 2003 Apr 1;118A(1):35–42. doi: 10.1002/ajmg.a.10011

Fig. 5.

Fig. 5.

An insertion within exon 2 in family DEN8. a: Amplification of a subfragment of exon 2 with primers hPAX9ex2a2F and hPAX9ex2a1R resulted in the expected 322-bp fragment and a novel 603-bp additional fragment only in the affected twin brothers II:3 and II:4 in family DEN8. b: The large fragment was sequenced and a 288-bp insertion detected (see Fig. 6). Primers, hPAX9InsF1 and hPAX9InsR, were designed to span the 5′and 3′ junctions of the insertion. Amplification with these primers resulted in a 308-bp fragment only in the affected brothers. c: Amplification with hPAX9ex2a2F and hPAX9InsR resulted in a 447-bp fragment only in II;3 and II:4.