Skip to main content
. 2018 Sep 14;194(1):54–66. doi: 10.1111/cei.13185

Figure 2.

Figure 2

Effective induction of cytomegalovirus (CMV)pp65‐specific CD8+ T cells by interferon dendritic dells/peptide and zoledronate plus α‐galactosylceramide (IFN DCs/P+Z+G) derived from patients with glioblastoma multiforme (GBM). (a) Each cell population was shown in the case of patient 1 possessing human leucocyte antigen (HLA)‐A*0201 and HLA‐A*0101, before culture. (b) After culture, IFN DCs from patient 1 induced CMVpp65‐specific CD8+ T cells most extensively when pulsed with HLA‐A*0201‐restricted P and Z+G in addition to the expansion of Vγ9γδT and Vα24NKT cells, in comparison with the other three different conditions (P, P+Z and P+G). (c) Expression levels of major histocompatibility complex (MHC) class I, programmed cell death ligand 1 (PD‐L1), PD‐L2, MHC class I‐related chain A (MIC‐A) and CD1d on the T98G tumour cell line established from a patient with GBM used as target cells are shown. (d) The effector cells including CD8+ T cells containing CMVpp65‐specific CD8+ T, Vγ9γδT and Vα24NKT cells (as shown in Fig. 2b with P+Z+G) killed the T98G tumour cell line more efficiently in a dose‐dependent manner, in comparison with the effector cells including mainly CD8+ T cells containing CMVpp65‐specific CD8+ T cells (as shown in Fig. 2b with P alone). (e) After culture, IFN DCs from patient 1 induced CMVpp65‐specific CD8+ T cells most extensively when pulsed with HLA‐A*0101‐restricted P plus Z+G, in addition to the expansion of Vγ9γδT and Vα24NKT cells, in comparison with the other three different conditions (P, P+Z and P+G).