Fig. 2. (a) Coupling of MAO-N activity to fluorescence in vivo. (b) FCM analysis of induced and non-induced E. coli cells containing the MAO-N expression construct, treated with H₂DCFDA and incubated with AMBA. (c) Comparison of fluorogenic dyes: FCM analysis of E. coli cells expressing MAO-N D5 stained with H₂DHDCF, C-H₂DHDCF or DHR and incubated with AMBA. (d) Optimisation of conditions for the expression of MAO-N D5 in E. coli: FCM analysis of E. coli cells incubated at 18 °C or 30 °C for 4 or 16 hours after inducing MAO-N D5 expression.