Table 3.
Expected and actual PCR products on gel are shown. Despite of deviation of amplicon sizes in both individual PCR products and home-made ladder containing mixture of PCR products, target species were clearly distinguishable from each other
| Target species | Expecetd PCR product size | Actual home-made ladder PCR product sizes on gel (approximately) | Actual indidividual PCR product size on gel (approximately) |
|---|---|---|---|
| Candida albicans | 1090 bps | 950 bps | 1200 bps |
| Candida africana | 901 bps | 800 bps | 1100 bps |
| Candida dubliniensis | 721 bps | 600 bps | 950 bps |
| Candida nivariensis | 588 bps | 500 bps | 700 bps |
| Candida orthopsilosis | 476 bps | > 400 bps | 576 bps |
| Candida glabrata | 386 bps | > 350 bps | 486 bps |
| Candida metapsilosis | 308 bps | ˜280 bps | 400 bps |
| Candida bracarensis | 214 bps | ˜200 bps | 300 bps |
| Candida parapsilosis | 112 bps | 100 bps | 250 bps |