Table 4.
Comparison of 9-plex PCR with CBS in-house MALDI-TOF database and sequencing of D1/D2 LSU rDNA
| Species | Multiplex PCR | MALDI-TOF | Sequencing |
|---|---|---|---|
| C. albicans (n = 128) | C. albicans (n = 128) | C. albicans (n = 128) | C. albicans (n = 128) |
| C. dubliniensis (n = 4) | C. dubliniensis (n = 4) | C. dubliniensis (n = 4) | C. dubliniensis (n = 4) |
| C. africana (n = 3) | C. africana (n = 3) | C. albicans (n = 3) | C. africana (n = 3)a |
| C. glabrata (n = 87) | C. glabrata (n = 87) | C. glabrata (n = 87) | C. glabrata (n = 87) |
| C. nivariensis (n = 2) | C. nivariensis (n = 2) | C. nivariensis (n = 2) | C. nivariensis (n = 2) |
| C. bracarensis (n = 2) | C. bracarensis (n = 2) | C. bracarensis (n = 2) | C. bracarensis (n = 2) |
| C. parapsilosis (n = 28) | C. parapsilosis (n = 28) | C. parapsilosis (n = 28) | C. parapsilosis (n = 28) |
| C. orthopsilosis (n = 6) | C. orthopsilosis (n = 6) | C. orthopsilosis (n = 6) | C. orthopsilosis (n = 6) |
| C. metapsilosis (n = 20) | C. metapsilosis (n = 20) | C. metapsilosis (n = 20) | C. metapsilosis (n = 20) |
a Similarity between C. albicans and C. africana using sequencing of D1/D2 LSU rDNAwas more than 99%
This library has been enriched with a diverse range of yeast species utilized in brewery