REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-ZO-1	Thermo Fisher	Cat No. 33–9100
Mouse monoclonal anti-zns-2	DSHB	AB 531907
Rabbit polyclonal anti-GFP	Thermo Fisher	A-11122
Goat polyclonal anti-GFP	(Venkiteswaran et al., 2013)
Sheep polyclonal anti-mCherry	Covance, this paper
Donkey anti-Goat 488	Thermo Fisher	A-11055
Goat anti-Rabbit 488	Thermo Fisher	A-11034
Donkey anti-Rabbit Cy3	Jackson Immunoresearch	Cat No. 711–166-152
Donkey anti-Goat Cy3	Jackson Immunoresearch	Cat No. 705–166-147
Donkey anti-Sheep 647	Jackson Immunoresearch	Cat No. 713–606-147
Donkey anti-Mouse Cy3	Jackson Immunoresearch	Cat No. 715–166-150
Donkey anti-Mouse 647	Jackson Immunoresearch	Cat No. 715–606-150
Sheep anti-DIG AP	Sigma Aldrich	SKU 11093274910
Bacterial and Virus Strains
NEB 5-alpha	New England Biolabs	Cat No C2987H
sw105	(Warming et al., 2005)
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
NBT	Sigma Aldrich	SKU 11383213001
BCIP	Sigma Aldrich	SKU 11383221001
Digoxigenin-labeled nucleotides	Sigma Aldrich	SKU 11277073910
Trizol	Thermo Fisher	Cat No 15596026
Paraformaldehyde	Sigma Aldrich	SKU P6148–500G
Proteinase K	Sigma Aldrich	SKU P6556–1G
Bovine Serum Albumin	Sigma Aldrich	SKU A8022–50g
Methycellulose	Sigma Aldrich	SKU 1424506–1G
Glycerol	Sigma Aldrich	SKU G7893–1L
Tricaine (Ethyl 3-aminobenzoate methanesulfonate)	Sigma Aldrich	SKU E10521– 50G
Low melt agarose (AquaPor HR)	National Diagnostics	Cat No EC-205 (100g)
Rhodamine 6G	Fischer Scientific	Cat No AC419010050
Alexa Fluor 594 Carboxylic Acid, tris(triethylammonium) salt	Thermo Fisher	A33082
BsrI	New England Biolabs	R0527S
Hpych4v	New England Biolabs	R0620L
AciI	New England Biolabs	R0551S
ApoI	New England Biolabs	R0566L
Taqαi	New England Biolabs	R0149L
Brefeldin A	Sigma Aldrich	B7651–5MG
Critical Commercial Assays
Thermoscript Reverse Transcriptase	Thermofisher	11146–024
TOPO TA cloning	Thermofisher	Cat No K461020
mMessage mMachine T7 kit	Thermofisher	AM1344M
mMessage mMachine SP6 kit	Thermofisher	AM 1340
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
fgf3/liat24149	(Herzog et al., 2004)
fgf10a/daetbvbo°	(Norton, 2005)
cldnb:lyn2GFP	(Haas and Gilmour, 2006)
hsp70:dn-fgfr1	(Halloran et al., 2000)
dusp6:d2EGFP	(Molina et al., 2007)
Tcf/Lef-miniP:dGFP	(Shimizu et al., 2012)
cdh2:cdh2-sfGFP-TagRFP	(Revenu et al., 2014)
cxcr4b:cxcr4b-Kate2-ires-GFP-CAAX	(Venkiteswaran et al., 2013)
fgfr1a/spdt3R705H	(Rohner et al., 2009)
prim:lyn2mCherry	this paper
hsp70:anos1b	this paper
hsp70:sec-mCherry	this paper
hsp70:fgf10a	this paper
fgf10a: GFP-fgf10a	this paper
fgf10a:secGFP	this paper
anos1a:GFP-anos1a	this paper
cxcr4b:H2A-mCherry	this paper
anos1ad5	this paper
anos1ad7	this paper
anos1bd5	this paper
Anos1bd79	this paper
Oligonucleotides
For DNA oligonucleotides, see Table S1
atoh1a morpholino: 5’- ATCCATTCTGTTGGTTT GTGCTTTT-3’	Gene Tools	Custom synthesis
Recombinant DNA
pCRII	Thermo Fisher	Cat No K461020
pDest-hsp70-SV40pA-tol2	(Kwan et al., 2007)
CH211–262I10 (fgf10a BAC)	BACPAC Resources Center
CH211–267B13 (anos1a BAC)	BACPAC Resources Center
DKEY-169F10 (cxcr4b BAC)	Source Bioscience
Golden Gate tAlEN and tAl Effector Kit 2.0	Addgene	ID 1000000024
Software and Algorithms
Simmune	https://www.niaid.nih.gov/research/simmune-project
Prism 7	Graphpad
FIJI (ImageJ v1.48)	https://fiji.sc/
Other