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Representative ESI-MS for the [M + 2H]2+ ions of a tryptic peptide of YTHDF2, SINNYNPK (a, b) and a tryptic peptide of YTHDF3, IGGDLTAAVTK (c, d) revealing the preferential binding of YTHDF2 and YTHDF3 toward the m1A-containing RNA probe in both forward (a, c) and reverse (b, d) SILAC experiments.
