Fig. 5.

Tan IIA-mediated mitochondrial fission triggers mitochondrial damage. a-b Mitochondrial ROS production was measured via flow cytometry. Mdivi-1 and Drp1 siRNA were used to prevent fission, which was used as the loss-of-function assay for mitochondrial fission. The mitochondrial fission agonist (FCCP) was added to the control group to activate mitochondrial fission, and this was used to mimic the effects of Tan IIA. c-d Antioxidant factors, including SOD and GPX, were quantified via ELISA in SW837 cells treated with Tan IIA. e-f Immunofluorescence assay for Smac. Blue fluorescence is from the DAPI probe, which stains the nucleus. g Caspase-9 activity was determined via ELISA. Mdivi-1 and Drp1 siRNA were used to prevent fission, which was used as the loss-of-function assay for mitochondrial fission. The mitochondrial fission agonist (FCCP) was added to the control group to activate mitochondrial fission, and this was used to mimic the effects of Tan IIA. h-i TUNEL staining to assess cell death. The number of TUNEL-positive cell was counted. *p < 0.05