Fig. 6.

Mitochondrial fission is implicated in Tan IIA-modulated SW837 cell migration and proliferation. a-b EdU staining for SW837 cells. The number of EdU positive cells was recorded. Mdivi-1, an antagonist of mitochondrial fission was administered into Tan IIA-treated cells to prevent fission, and this was used as a loss-of-function assay for mitochondrial fission. Mitochondrial fission agonist (FCCP) was added into the control group to activate mitochondrial fission, which was used to mimic the effects of Tan IIA. c-e Immunofluorescence analysis for the proliferation-related proteins. Mdivi-1 and FCCP were administered to perform the loss- and gain-of-function assays to assess mitochondrial fission, respectively. f. ATP production was detected via ELISA. g-i Immunofluorescence assay for F-actin and filopodium. The length of filopodium was recorded. Mdivi-1 and FCCP were administered to perform the loss- and gain-of-function assays for mitochondrial fission, respectively. j-k Transwell assay in the presence of Mdivi-1 and Z-VAD-FMK. *p < 0.05