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. 2018 Sep 12;2018:2190657. doi: 10.1155/2018/2190657

Table 1.

Culture media compositions.

Component BM OM AM Manufacturer
Dulbecco's Modified Eagle Medium/Ham's Nutrient Mixture F-12 (DMEM/F-12) Thermo Fisher Scientific, Waltham, MA, USA
GlutaMAX 1% 1% 1%
Insulin 100 nM
Human serum (HS) 5% 5% 5% PAA Laboratories GmbH, Pasching, Austria
Penicillin/streptomycin 1% 1% 1% Lonza, Basel, Switzerland
L-Ascorbic acid 2-phosphate 200 μM Sigma-Aldrich, Saint Louis, MO, USA
β-Glycerophosphate 10 mM
Dexamethasone (DEX) 5 nM 1 μM
Pantothenate 17 μM
Biotin 33 μM
3-Isobutyl-1-methylxanthine (IBMX) 0.25 M