Table 1.
Component | BM | OM | AM | Manufacturer |
---|---|---|---|---|
Dulbecco's Modified Eagle Medium/Ham's Nutrient Mixture F-12 (DMEM/F-12) | Thermo Fisher Scientific, Waltham, MA, USA | |||
GlutaMAX | 1% | 1% | 1% | |
Insulin | — | — | 100 nM | |
Human serum (HS) | 5% | 5% | 5% | PAA Laboratories GmbH, Pasching, Austria |
Penicillin/streptomycin | 1% | 1% | 1% | Lonza, Basel, Switzerland |
L-Ascorbic acid 2-phosphate | — | 200 μM | — | Sigma-Aldrich, Saint Louis, MO, USA |
β-Glycerophosphate | — | 10 mM | — | |
Dexamethasone (DEX) | — | 5 nM | 1 μM | |
Pantothenate | — | — | 17 μM | |
Biotin | — | — | 33 μM | |
3-Isobutyl-1-methylxanthine (IBMX) | — | — | 0.25 M |