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. 2018 Sep 12;2018:6285813. doi: 10.1155/2018/6285813

Figure 5.

Figure 5

Effect of serum from endometriosis patients on macrophage polarization. Flow cytometric analysis (a) showed that treatment with serum from endometriosis patients resulted in an increased percentage of CD163+ macrophages in response to LPS stimulation as compared with the control (b). The mRNA levels of CD86 (c), CD163 (d), IL-12 (e), and IL-10 (f) were detected by RT-PCR. Immunohistochemical staining analysis (g) revealed significantly increased intensity of CD163 staining in macrophages treated with serum from endometriosis patients compared with that in controls (h). LPS: lipopolysaccharide. n = 12, ∗ P < 0.05, ∗∗ P < 0.01.