Figure 5.

8 μ M of (+)-UA induced autophagy via inhibition of mTOR. (a) Western blot: the treatment of HCT116 cells with 8 μM of (+)-UA mediated an autophagy with a complete autophagic flux in 48 hours rather than in 24 hours. (b) Quantitative analysis: ^aP < 0.05, ^bP < 0.05, ^cP < 0.05, ^dP < 0.05, and ^eP < 0.05, versus CTR. (c) Western blot: (+)-UA-mediated autophagy was dependent on inhibition of mTOR. (d) Quantitative analysis: ^aP < 0.05, ^bP < 0.05, ^cP < 0.05, ^dP < 0.05, and ^eP < 0.05, versus scramble siRNA, ^fP < 0.05, ^gP < 0.05, ^hP < 0.05, ⁱP < 0.05, and ^jP < 0.05, versus (+)-UA.