FIGURE 3.

Overexpression of MYCT1 inhibits cell proliferation and induces cell cycle arrest in HL-60 and KG-1a cells. Cells proliferation was measured by CCK-8 assay in HL-60 (A) and KG-1a (B) cells at 24 h, 48 h, 72 h and 96 h after lentiviral delivery. (C–F) MYCT1 overexpression-induced cell cycle arrest in HL-60 and KG-1a cells. Changes in cell cycle distribution of MYCT1-overexpressing HL-60 (C) and KG-1a (D) cells were determined by FACS analysis, and the proportions of cells in G₀/G₁, S and G₂/M phase were calculated (D,F). (G–J) The expression levels of cell cycle regulatory proteins in HL-60 (G,H) and KG-1a (I,J) cells were examined by Western blot analysis. ^∗P < 0.05 and ^∗∗P < 0.01 versus HL-Lv-NC or KG-Lv-NC cells.