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. 2018 Sep 19;9:2095. doi: 10.3389/fimmu.2018.02095

Figure 2.

Figure 2

Vibrio vulnificus rtxA mutant was defective in inducing the secretion of Th17 polarization-inducing cytokines. (A) Total RNA was extracted from DCs infected with WT and mutant ahpCl, rtxA, and vvhA mutant Vibrio vulnificus at an MOI of 1 for 30 min. The expression levels of mRNAs for several Th17-polarizing cytokines were determined by RT-PCR. (B) DCs were infected for 60 min with WT and mutant ahpCl, rtxA, and vvhA mutant Vibrio vulnificus at an MOI of 1. The protein levels of IL-1β, IL-6, and IL-23 in the supernatants collected were determined by ELISA. The data shown in (A) are representative of three independent experiments, and bar graphs represent the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005 as determined by Student's t-test for pairwise comparisons. WT, wild type; ahpCl, ahpCl mutant; rtxA, rtxA mutant; vvhA, vvhA mutant.