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. 2018 Sep 19;9:2095. doi: 10.3389/fimmu.2018.02095

Figure 5.

Figure 5

Vibrio vulnificus hlyU mutant reproduced the results observed for rtxA mutant. (A) DCs were infected with WT and hlyU, rtxA, and smcR mutant V. vulnificus at an MOI of 1 for 30 min. (B) Total RNA was extracted from DCs infected with WT and hlyU, rtxA, and smcR mutant strain. (C) DCs were infected for 60 min with WT and hlyU, rtxA, and smcR mutant strain at an MOI of 1. (D) DCs were infected with WT and hlyU, rtxA, and smcR mutant strain at an MOI of 10 for 60 min. After 20 h, DCs were co-cultured with naïve CD4+ T cells isolated from lymph nodes for 3 days in the presence of anti-CD3ε and anti-CD28 mAbs, followed by the flow cytometric analysis of CD4 and IL-17 expressions. The data shown in (A,D) are representative of three independent experiments, and bar graphs represent the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005 as determined by Student's t-test for pairwise comparisons. WT, wild-type; hlyU, hlyU mutant; rtxA, rtxA mutant; smcR, smcR mutant.