Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 24;28(5):285–296. doi: 10.1089/nat.2018.0734

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ruchi Jain et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

FIG. 2. — Endogenous miRNAs can be utilized to suppress protein expression from synthetic modified mRNAs in specific cells. (a) Schematic representation of Luc mRNA with a miRts in the 3′ UTR. (b–d) miRts incorporation in modified mRNA leads to suppression of encoded protein in specific cells. RLUs 6 h after L2000-mediated transfection of 1-methyl-pseudouridine (m¹Ψ) mRNA encoding Luc in (b) HeLa, (c) primary human hepatocytes, and (d) RAW 264.7 macrophages. Each datum in the bar graph is an average of three biological samples, and the error bars represent standard deviation. Luminescence from cells with miRts-containing mRNAs was compared with cells with CTRL mRNA, and P values were generated by Prism using the unpaired, two-tailed t-test. ***P < 0.001, ****P < 0.0001. miRNA, microRNA; RLU, relative luminescence units; UTR, untranslated region.