FIG. 3.
Time-dependent effect of dexamethasone on RET mRNA. Quantification of results obtained by Northern blot analysis (not shown). TT cells were treated in triplicate with 10−6 M dexamethasone for 6, 12, 24, 48, and 72 h. RET mRNAs were again quantitated, adjusted against 36B4 species for loading, and expressed as a percentage of control lanes. Cells at time zero were used as a control, because RET expression had not been found to alter significantly over 72 h in previous experiments (data not shown). Significant differences when compared to control results are shown: *p < 0.05, **p < 0.01, ***p < 0.005.