FIG. 5.

Effect of cycloheximide on RET mRNA levels and their dexamethasone-induced inhibition. (A) Northern analysis. TT cells were treated for the time shown with 10^-8 M dexamethasone (D), 20 μg/ml cycloheximide (C), or both substances (D/C) as described in Materials and Methods. Representative results from duplicate samples are shown. (B) Quantification of results obtained from Northern analysis. Each of the 7.0-, 6.0-, 4.5-, and 3.9-kb RET species are assessed in separate panels here. The effects of dexamethasone alone (D), cycloheximide (C), or both substances (D/C) are expressed as a percentage of the mean vehicle-treated control lanes (Et) after correction for loading with 36B4.