FIG. 1.

Effect of SRC-1 overexpression on AhR-mediated transactivation in COS-1 and Hepa 1c1c7 cells. Hepa 1c1c7 cells (A) and COS-1 (B) were cotransfected with increasing amounts of pSG5-SRC-1a, 100 ng of DRE-driven luciferase gene containing plasmid, pGUDLUC 6.1, and pCMV/LacZ (100 ng), and empty plasmid. COS-1 cells were also cotransfected with 100 ng pcDNA3/βmAhR where indicated. Luciferase activity was measured following a 12-h induction with 10 nM TCDD or carrier solvent and normalized to β-galactosidase activity. Each transfection was performed in triplicate. For statistical analysis, the values from the SRC-1 transfected samples were compared with those of SRC-1 nontransfected samples within each cell line. Asterisks indicate that statistically significant differences were observed (p < 0.05).