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. 2018 Sep 6;159(10):3581–3595. doi: 10.1210/en.2018-00559

Figure 4.

Figure 4.

Knockdown of TRPS1 increases PR occupancy and increases the expression of PR-regulated genes. T47D cells were transfected with siRNA to either control (sic) or TRPS1. After transfection, cells were treated with VC, 10 nM R5020, 1 μM TPA, or R5020+TPA for 24 hours. (A) Nuclear extracts were harvested and immunoblotted for TRPS1 and actin. (B) ChIP assay was done with anti-PR and IgG as control after 30 minutes of treatment. (C) RNA was extracted and real-time PCR analysis was performed. Primer sequences are in an online repository (26). The data are represented as fold change of VC treatment (dotted red line), mean ± SEM from three independent experiments. **P < 0.01; ***P < 0.0005; ****P < 0.0001. (D) RNA was extracted and real-time PCR analysis was performed. Primer sequences are from previously published data (24). The data are represented as fold change of VC treatment (dotted red line), mean ± SEM from three independent experiments. *P < 0.05. (E) After 30 minutes of hormone treatment, ChIP was done with antip300 or IgG as control. All data are represented as the mean ± SEM from three independent experiments. *****P < 0.05; **P < 0.001; ***P < 0.0005; ****P < 0.0001.