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. 2018 Sep 19;9:1395. doi: 10.3389/fpls.2018.01395

FIGURE 1.

FIGURE 1

Phylogenetic relationships, domains, in silico expression profiling, and gene structure of ARFs in wheat. (A) Phylogenetic tree of TaARFs constructed from the complete alignment of 68 wheat ARF protein sequences by the neighbor-joining method with 1000 bootstrap replicates using MEGA 6.0. The bootstrap scores are indicated on the nodes, and the 23 TaARF members, all of which are coded by homeoalleles, are indicated in blue or pink boxes. The branch of paralogous TaARF members is shown in bold. (B) Conserved domains of TaARFs. DBD, DNA-binding domain; CTD, C-terminal dimerization domain; MR, middle region; S, serine; P, proline; G, glycine; L, leucine; Q, glutamine. (C) In silico expression profiling of TaARF homeoalleles in different organs at different growth stages of Chinese Spring wheat. The expression data were generated from the expVIP database (http://www.wheat-expression.com/). The color scale at the top represents the expression values: black indicates low levels of transcript abundance, and yellow indicates high levels. R, root; L/S, leaf/stem; Sp, spike; G, grain; Se, seedling stage; Ve, vegetative stage; Re, reproductive stage. (D) Exon/intron structures in the CDS of TaARF genes. The number of AS variants is listed before each gene. For genes that represent predicted AS variants, the variant with the highest in silico expression level has been selected for the gene structure analysis. Exons are represented by black boxes and introns by black lines. The size of exons and introns can be estimated using the scale at the bottom.