FIG. 11.

Analysis of PE molecular species in the ileum of mice exposed to TBI. C57BL/6NTac mice were exposed to TBI at a dose of 9.5 Gy. After that, mice were sacrificed, ileum was isolated, lipids were extracted, and detailed normal-phase LC-MS/MS analysis of PE molecular species was performed (75). Analysis of nonoxidized PE metabolites revealed 115 molecular species of PE, 58 of which were confirmed by fragmentation and MS/MS analysis (Table 1). Analysis of LC-MS data was performed by using software package Compound Discoverer™ (ThermoFisher Scientific). Typical full spectrum of PE obtained from the mouse ileum (A). Scatter plot of changes in the levels of nonoxygenated (upper panels) and oxygenated (lower panels) PE species (log₂ [fold-change] vs. significance −log₁₀ [p-value] [n = 4]) (B). The number of PE oxygenated species detected in mouse ileum after TBI are shown in (C). PE, phosphatidylethanolamine. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars