FIG. 14.

Accumulation of PE oxygenated species in the ileum of mice exposed to TBI (9.5 Gy). The contents of mono-, di-, tri-, and tetra-oxygenated PE species in the ileum of naive mice and mice exposed to TBI (A). The ileum lipids were analyzed by normal-phase LC-MS by using a Q-Exactive mass spectrometer using software package Compound Discoverer (ThermoFisher Scientific) with an in-house generated analysis workflow and oxidized phospholipid database. Data are mean ± SD, n = 5, *p < 0.05 versus naive (0 day) nonirradiated mice. Note: The elevated levels of oxygenated PE species in the ileum were detected on days 4–7 after exposure irradiation. The significant level of PE-18:0/20:4-OOH, a ferroptotic cell death signal, was detected in the ileum on day 5 after irradiation (B). Data are mean ± SD, n = 5. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars