FIG. 7.
LC-MS identification of oxygenated TLCL species formed by the peroxidase activity of cyt c/CL complex in the presence of H2O2. TLCL (25 μM) was incubated in the presence of cyt c (5 μM) in 25 mM HEPES buffer pH 7.4 containing 100 μM DTPA. TLCL oxidation products were analyzed by normal-phase LC-MS by using a Q-Exactive mass spectrometer (ThermoFisher Scientific) (155). Shown are full spectra of oxygenated and nonoxygenated (inset) TLCL species (A). MS/MS analysis revealed the presence of TLCL species with one (B), two (C), three (D), four (E), and five (F) oxygens. MS/MS, mass spectrometry; TLCL, tetra-linoleyl-CL. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars