Figure 4.

Quantitative profiling of bile acid in plasma from random-fed Gcgr^−/−:Glp2r^−/−double knockout mice and their single knockout and wild-type littermates. Panel A: Bile acid composition analysis representing the percentage of the major BA which contribute to the BA pool in plasma. Total average concentrations of bile acids in plasma were: Gcgr^+/+:Glp2r^+/+ = 2947.6 nM, Gcgr^+/+:Glp2r^−/− = 4013.2 nM, Gcgr^−/−:Glp2r^+/+=24942.5 nM and Gcgr^−/−:Glp2^−/−=13513.9 nM. Panel B: Heat map summarizing the concentrations of the different molecular species of BA quantified in plasma samples. The color code represents the log2 transformation of the corresponding mean BA concentration values. For both Panels A & B n = 8 mice per genotype, combined from 2 independent mouse cohorts. T-conjugated (taurine-conjugated BA), G-conjugated (Glycine-conjugated BA), MCA (sum of αMCA, βMCA and ωMCA concentrations). Scatter plots for the BA illustrated in the heat map are presented in Supplementary Figure 1.