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. 2018 Jun 18;16:1–11. doi: 10.1016/j.molmet.2018.06.008

Figure 3.

Figure 3

Nutritional challenges reprogram daily variation of human skeletal muscle metabolome. (A) Number of muscle metabolites affected by diet or time. (B) Separation of samples based on the diet and collection time by PCA. (C) Heatmaps display significantly gradient muscle metabolites exclusively in pre (left panel) and post HFD (middle panel), and in both pre- and post-HFD (right panel). (D) Pie chart shows the number and ratio of significantly gradient metabolites in the muscle under HFD condition. Numbers within the pie chart indicate number of gradient metabolites. Gradient, metabolites significantly different between morning and evening, Morning, metabolites being significantly higher in the morning, and Evening, metabolites being significantly higher in the evening. (E) Heatmaps display gradient muscle metabolites exclusively in pre- (left panel) and post-HCD (middle panel), and in both pre and post HCD (right panel). (F) Pie chart exhibits the number of gradient metabolites in the muscle under HCD treatment. (G) Volcano plot exhibits the significance of gradient muscle metabolites under pre- (open circle) and post- (closed circle) HFD condition. The x-axis and y-axis represent the fold change (evening/morning) and significance of gradient muscle metabolites, respectively. (H) Top 3 gradient muscle metabolites unique to pre-HFD (top) and post-HFD (bottom). ** and *** indicates P < 0.01 and P < 0.001 between morning and evening, §§ indicate P < 0.01 between pre and post in the morning, ††† indicate P < 0.001 between pre and post in the evening. (I) Volcano plot exhibits the significance of gradient muscle metabolites under pre- (open circle) and post- (closed circle) HCD condition. (J) Top 3 gradient muscle metabolites unique to pre-HCD (top) and post-HCD (bottom). ** and *** indicates P < 0.01 and P < 0.001 between morning and evening, §, §§ and §§§ indicate P < 0.05, P < 0.01 and P < 0.001 between pre and post in the morning, ††† indicate P < 0.001 between pre and post in the evening. (K) Histogram shows the biological classification of gradient metabolites in the muscle under HFD (left) and HCD (right) conditions. Orange and blue bars indicate the number of metabolites being higher in the morning and evening, respectively, within each classification. Two-way ANOVA compares the mean differences of metabolites levels between morning and evening. Adjusted p-value cutoff of 0.05 was used. N = 8 biological replicates in each time of day were subjected to the metabolomics analysis.