Table 2.
Kinetic parameters of bradykinin hydrolysis by different enzymes.
| Enzyme | Km (μmol/L) | kcat (min−1) | kcat/Km (min−1 μmol/L−1) | References |
|---|---|---|---|---|
| ACE (kininase II) | 0.18, 1 | 500, 600 | 500, 3667 | (25, 26) |
| Neprilysin (neutral endopeptidase 24.11) | 34, 92, 120 | 1500, 4771, 6364 | 40, 44, 69 | (3, 26, 27) |
| NEP2 | 2 | 150 | 75 | (3) |
| Aminopeptidase P | 21, 76, 280 | 720, 1560, 2280 | 8, 21, 34, | (5, 28, 29) |
| Carboxypeptidase N (kininase I) | 19 | 58 | 3 | (30) |
| Carboxypeptidase M | 16 | 147 | 9.2 | (31) |
| Neutral endopeptidase 24.15 | 4.9 | 89 | 18 | (32) |
| Endothelin converting enzyme-1* | 340 | 1380 | 4.1 | (33) |
| Endothelin converting enzyme-2† | 27.4 | 348 | 12.7 | (34) |
ACE, angiotensin converting enzyme; NEP2, neprilysin homolog membrane metalloendopeptidase-like 1.
*
pH 6.5;
†
pH 5.5.